Journal: Journal of Sport and Health Science

Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice

doi: 10.1016/j.jshs.2025.101095

Figure Lengend Snippet: Impact of high fat feeding and voluntary exercise on left ventricle morphology in male mice. (A) Representative images of Picrosirius red staining for measurement of interstitial and perivascular fibrosis with 20× magnification. (B) Representative images for Hematoxylin and Eosin for measurement of cardiomyocyte width and area with 40× magnification. (C) Representative images of Oil red O staining for measurement of interstitial lipid deposition with 40× magnification. Quantitative analysis of percent area of (D) interstitial and (E) perivascular fibrosis expressed as fold change from sedentary chow group. Quantitative analysis of (F) cardiomyocyte width and (G) cardiomyocyte area. (H) Quantitative analysis of percent area of lipid deposition expressed as fold change from sedentary chow group left ventricle mRNA expression of fatty acid transporters (I) FABP3, (J) CD36, and hypertrophy markers (K) β-MHC and (L) ANP in male VET or sedentary mice fed an HFD or chow diet. Values were calculated relative to 18S housekeeper. Analysis was performed using two-way analysis of variance with Tukey’s post hoc test for multiple comparisons. Data are expressed as mean ± standard error of the mean. (D–H) n : 8–12 per group. (I–L) n : 8–9 per group. * p < 0.05, **** p < 0.0001. ANP = atrial natriuretic peptide; β-MHC = beta myosin heavy chain; CD36 = platelet glycoprotein 4; FABP3 = fatty acid-binding protein 3; HFD = high fat diet; Myh7 = moysin heavy chain 7; VET = voluntary exercise training.

Article Snippet: The following TaqMan assay gene transcripts were used: fatty acid-binding protein 3 (FABP3, Mm02342495_m1), platelet glycoprotein 4 (CD36, Mm00432403_m1), beta myosin heavy chain (β-MHC, Mm00600555_m1), atrial natriuretic peptide (ANP, Mm01255747_g1), OPA1 (Mm01349707_g1), DRP1 (Mm01342903_m1), robosomal18S (18S, Mm03928990_g1).

Techniques: Staining, Expressing, Binding Assay

Journal: bioRxiv

Article Title: Bloodstream-associated Salmonella Typhimurium and Enteritidis iNTS pathovariants hyper-replicate in human macrophages

doi: 10.64898/2026.01.28.702237

Figure Lengend Snippet: (A) Representative phase contrast images of THP-1 cells untreated versus THP-1 cells differentiated with Condition A (PMA 10 ng/mL for 24 h), Condition B (PMA 10 ng/mL for 24 h followed by a 24 h recovery in fresh media), Condition C (PMA 10 ng/mL for 48 h) and Condition D (PMA 50 ng/mL for 48 h). (B) Representative flow cytometric analysis of THP-1 cells and macrophage-induced cells stained using anti-CD11b, anti-CD14 and anti-CD36 antibodies. (C) Median fluorescence intensity (MFI) of CD11b, CD14 and CD36. Data are displayed as mean ± SD from n ≥ 3. Statistical analyses performed using the Brown-Forsythe and Welch ANOVA test and the Dunnet T3 multiple comparison post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Macrophage surface markers expression was assessed via flow cytometry by staining the cells with anti CD11b (FITC, #130-110-552, Miltenyi Biotec), anti CD14 (APC, #130-110-520, Miltenyi Biotec), anti CD36 (APC-Vio770, #130-110-743, Miltenyi Biotec), anti CD86 (PerCP-Vio700, #130-116-267, Miltenyi Biotec), anti HLA-DR (VioGreen, #130-111-795, Miltenyi Biotec), anti CD163 (PE, #130-112-128, Miltenyi Biotec), anti CD206 (VioBlue, #130-127-809, Miltenyi Biotec) according to manufacturer’s instructions.

Techniques: Staining, Fluorescence, Comparison

Journal: Genes & Diseases

Article Title: cTnIR193H restrictive cardiomyopathy mice satisfy high-energy metabolic demands through regulating glucose metabolism

doi: 10.1016/j.gendis.2025.101784

Figure Lengend Snippet: GLUT4 and CD36 expression levels. (A) GLUT4 and CD36 mRNA expression levels (WT, n = 4; cTnI193His-M, n = 3). (B) Immunohistochemical staining of GLUT4 and CD36 in heart tissue ( n = 3). Photographs were taken at 400× using an electron microscope. (C) CD36 and GLUT4 protein levels ( n = 3). (D) Statistical analysis of western blotting results for the detection of protein expression. (E) GLUT4 immunofluorescence staining on the cell membrane ( n = 3). (F) CD36 immunofluorescence staining on the cell membrane ( n = 3). Photographs were captured at 400× using a confocal laser. ∗ P < 0.05; ∗∗ P < 0.01.

Article Snippet: Western blotting analysis was used to detect the expression levels of GLUT4, CD36, PI3K, Akt, and p-Akt using specific antibodies (GLUT4, 1:2000, Proteintech, 66846-1-Ig, China; CD36, 1:500, Wanleibio, WL02390, China; PI3K, 1:1000, Wanleibio, WL03380, China; Akt, 1:500, Wanleibio, WL0003b, China; p-Akt, 1:500, Wanleibio, WLP001a, China).

Techniques: Expressing, Immunohistochemical staining, Staining, Microscopy, Western Blot, Immunofluorescence, Membrane